Flow-Seq of GFP library with intron in HeLa cells - SRA

SRX7391409: Flow-Seq of GFP library with intron in HeLa cells
1 ILLUMINA (Illumina MiSeq) run: 1.3M spots, 748.3M bases, 359.9Mb downloads

Design: To prepare Flow-Seq libraries, cells were FACS sorted by GFP fluorescence into eight bins, followed by genomic DNA isolation and PCR with primers specific to GFP 5'- and 3'-UTR sequences

Submitted by: University of Edinburgh

Study: Splicing buffers suboptimal codon usage in human cells

PRJNA596086 • SRP237851 • All experiments • All runs

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We measured the effects of splicing on expression in a panel of synonymous variants of GFP and mKate2 reporter genes that varied in nucleotide composition. We found that splicing promotes the expression of adenine and thymine (AT)-rich variants by increasing their steady-state protein and mRNA levels, in part through promoting cytoplasmic localization of mRNA. Splicing had little or no effect on the expression of guanine and cytosine (GC)-rich variants.

Sample:

SAMN13612109 • SRS5839594 • All experiments • All runs

Organism: synthetic gene

Library:

Name: CM9b_Bin2

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: SYNTHETIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 1.3M spots, 748.3M bases, 359.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR10714533	1,251,318	748.3M	359.9Mb	2021-01-01

ID:: 9677131

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